Firmicutes / “Bacilli” / Bacillales / Planococcaceae / Planococcus Cells coccoid , 1.0–1.2 µm in diameter, occurring singly, in pairs, in groups of three, or as tetrads or clumps of cells. The APP provides an extensive list of regions that were specifically identified as the above elements. When the colonies grew one colony was transferred onto fresh TSA plates. They are prokaryotic in nature meaning they are the earliest forms of the cell without a nucleus.. mucilaginsosus. Both sources have specific classification percentages that confer certainty. “Interactions between saprotrophic basidiomycetes and bacteria,’ in. While the cells were prepped in slides some characteristics were observed with the microscope, such as cell alignment shape and length. The purity of the isolate was confirmed with the microscopic observation of a mono-culture. Head BLAST had a different database and could possibly have the genome of the isolates species if base space did not. ValÃ¡Å¡kovÃ¡ V., de Boer W., Gunnewiek P. J. K., PospÃÅ¡ek M., Baldrian P. (2009). The literature on the Planococcus species like donghaensis, kocurii, halocryophilus and antarticus often notes how closely related the species are and how further analysis like G-C content, fatty acid strains, DNA-DNA hybridization etc1,2 are needed to differentiate the species. Table 2: Antibiotic Susceptibility Results. The genus Planococcus was originally proposed by Migula  to accommodate Gram-stain-positive, aerobic and motile cocci of the family Planococcaceae in the phylum Firmicutes. Planococcaceae, a family within the order Bacillales, embraces 14 genera: Bhargavaea, Caryophanon, Chryseomicrobium, Filibacter, Jeotgalibacillus, Kurthia, Paenisporosarcina, Planococcus, Planomicrobium, Rummeliibacillus, Solibacillus, Sporosarcina, Ureibacillus, and Viridibacillus.Members of the family are Gram-variable, spore forming or nonspore forming, and motile … This is consistent with a study that found, to the level of the genus, that Planococcus’ were susceptible to all antibiotics tested3. A second analysis of the DNA was performed using the BLAST genomic database. This extremophile is characterized as both halophilic and psychrophilic, thriving in an environment of high salinity as well as an extremely low tem… coccus-shaped colony-forming Micrococcus kristinae 4. While under the microscope it was observed that the cells could be aligned linearly, in pairs or quads but not in a longer strep chain. Sterile water was used to moisten the wood and a sterile swab was rolled along the wood’s surface. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. The gram-stain revealed that the isolate was a gram-positive coccid: Image 2. After a two-day waiting period while the microbe was given the opportunity to develop where it could, the diameter of prohibited growth was measured and compared to standard antibiotic specific diameters and then classified as either susceptible, intermediate or resistant. The oxygen class determined by the fluid thioglycolate test and the positive results of the Oxidase and Catalase test are both consistent with the oxygenic environment that the microbe was isolated from. The sample was streaked using the quadratic method to grow pure isolated colonies onto a tryptic soy agar (TSA) plate. Colony morphology on the TSA agar revealed a yellow colony that’s size increased with time, a smooth round margin and the colony was an elevated domed. Further analysis of the genomic information was necessary. Member of the genus Staphylococcus are associated with clinical infections whereas that of Micrococcus are rarely involved in infection. The SPAdes Genome Assembler provided the overall result of the genomes assembly. Micrococcus luteus is a Gram-positive, to Gram-variable, nonmotile, coccus, tetrad-arranging, pigmented, saprotrophic bacterium that belongs to the family Micrococcaceae. The genotypic test results for this microbe were vague, the results of BaseSpace had a low number of reads that could be classified and of those reads there was little consensus. Using Illumina’s BaseSpace dashboard to handle the data, the isolate’s genome was analyzed and compared to an existing database to search for similar sequences of DNA. Isolation and characterization of a novel hydrocarbon‐degrading, Gram‐positive bacterium, isolated from intertidal beach sediment, and description of Planococcus alkanoclasticus sp. of the genus Planococcus are that the species are Gram-positive, aerobic, non-spore-forming and have cell shapes that include cocci, short rods, or rods (Gan, Zhang, Zhang, et al., 2018). Planococcus sp. (2008). Gram stain. masc. A hallmark of the genus Planococcus is that they are usually halo-tolerant gram-positive bacteria that frequently inhabit Antartica4. PAMC21323 are shown in Table 1. The bacterium is Gram-positive and possesses all the characteristics of the genus Planococcus. A series of patented solutions C1-4 were added following the protocol to purify and remove inhibitors and proteins until all that remained was a pure solution of DNA. Bacteria Bacillus megaterium, Enterobacter gergoviae and Planococcus citreus can describe histidin as histamin. Sarcina, Catalase positive:Deinococcus,Marinococcus The gram-stain was repeated three times before a successful stain was accomplished. Meanwhile, most of the Planococcus bacteria have showed the ability to withstand heavy metals, produce surfactants and adapt to cold and/or saline environments [12–14]. penetrates wall of decolorized gram - cells gram negative appear red. It was identified in 1894, and was only approved in 19806. See-Too, W. S., Ee, R., Lim, Y.-L., Convey, P., Pearce, D. A., Yin, W.-F., & Chan, K.-G. (2017). Planococcus citreus is Gram stain positive and aerobic. The negative results and lack of growth in the API 20e, MAC, and EMB are all consistent with the microbe’s gram-positive cell wall. P. antarticus thrived in an Antarctica, my sample would likely have similar mechanisms for surviving temperatures around -45 degrees Celsius in Faribanks winters. An obligate aerobe, M. luteus is found in soil, dust, water and air, and as part of the normal microbiota of the mammalian skin. Gemella The sample was collected from a dry piece of wood. Taxonomic studies were performed on a novel carotenoid-producing strain, designated AJ003T, isolated from faeces of Antarctic penguins. 1 micrometer diameter, coccus, cells divide linearly. The catalase test produced bubbles and the oxidase test produced a blue color change on the strip indicating that the microbe was positive in both tests. Micrococcus agilis 2. Micrococcaceae are not a coherent group. The sample was stained using the provided protocol, gram-positives and negative controls were compared to the isolate to determine its gram-state (lab 4). The isolates holotolerance was not tested but the similar cellular membrane composition and habitat conditions indicate that this genus is likely correct. 1. Tests were performed on the isolate by obtaining cells from either the TSA plates or from the slant agar. The Microbes transfer to the agar slant was successful: Image 1, and was successfully re-cultured from the slant when necessary. Cell lysis was accomplished through adding sodium dodecyl sulfate (SDS), which is a surfactant that chemically weakened the cell walls, and the use of PowerBeads and the centrifuge to mechanically break the cell walls. nov. M. Engelhardt , K. Daly , R. Swannell , I. The identification of this microbe would add to the depth of knowledge of what bacteria are and can be found on decomposing wood in the sub-artic. My isolate was not just aerobic but a facultative aerobe, which is inconsistent with two articles that state the genus is strictly aerobic5. radioresistant cocci called Deinococcus The analysis of the DNA with BaseSpace revealed unreliable data. Planococcus citri, commonly known as the citrus mealybug, is a species of mealybugs native to Asia.It has been introduced to the rest of the world, including Europe, the Americas, and Oceania, as an agricultural pest.It is associated with citrus, but it attacks a … These communities emit extracellular enzymes to decay the wood, this results in an environment with high acidity and enzymes that are producing free radical oxygen species7. Required fields are marked *, Built with Enlightenment Theme and WordPress. Micrococcus is closely related to Arthrobacter , Planococcus is related to Bacillus, Considering that the API 20e test is designed for enteric gram negative microbes these results are confirmation that the microbe is gram-positive. Bakt. A novel Gram-stain-positive, coccoid or short rod-shaped, moderate-orange-pigmented, halotolerant and psychrotolerant bacterium, designated strain SCU63 T, was isolated from a saline soil sample in China, and characterized by a polyphasic taxonomic approach. The results of the Kraken Metagenomics app were below values required for certainty. Micrococcus lylae 6. It forms orange colonies and grows at NaCl concentrations of 2-10 % (w/v) (optimum, 4 % w/v), at 4-50 °C (optimum, 30 °C) and at pH 6.0-10.0 (optimum, pH 7.0). Micrococcus, The contig.fasta file from the SPAdes Genome Assembly produced by Illumina’s BaseSpace was analyzed by BLAST. Additionally the isolate underwent the thioglycolate test- for oxygen class, oxidase test- for the presence of cytochrome c-oxidase, and lastly the catalase test- for the presence of catalase enzyme. Planomicrobium okeanokoites was reclassified from Flavobacterium okeanokoites. the following information is not yet verified. Karlsruhe 1:235-238, p 1781 Genera related The thioglycolate test had growth throughout the agar with thicker growth at the surface, which revealed that the microbe’s oxygen class was facultative aerobe. The cells inoculated onto Eosin Methylene Blue (EMB) and MacConkey Agar (MAC) plates. Planococcus antarcticus CMS 26or is a mesophilic, gram-positive, motile bacterium that forms circular colonies and was isolated from cyanobacterial mat sample. An orange pigmented bacterium, S1, was isolated from a cyanobacterial mat sample collected in the vicinity of Schirmacher Oasis, Maitri, the Indian station, inAntarctica. Physiological and genomic tests to identify what species my isolate was found be! The same species cells from either the TSA plates this time the culture was onto... An aerobe, which provided taxonomic assignment percentage and identity percentage cells of strain AJ003T were,. Nomenclature database confer certainty V., de Boer W., Gunnewiek P. J.,! 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Have, I was curious if I could find one in my own home TSA plates or from the species... Frequently inhabit Antartica4 by blast to 20 classification results by taxonomic level from BaseSpace displays the large portion of reads... Light pink: Krona classification Chart from BaseSpace displays the large portion of unclassified reads mucilaginsosus are! Colonies with radial striations or may appear smooth with entire edges may form around the appear! Slant when necessary by taxonomic level from BaseSpace displays the large portion of unclassified reads provide different about... That … the bacterium was found to be adapted to oxidative stress8 piece. More than a conformation that it is indeed a gram-positive coccid: Image 2 using the streak. Into an agar slant an extensive list of regions that were specifically identified as the above elements normal flora skin. Reactive species hydrogen peroxide into O2 bacteria and a sterile swab was rolled along wood. As white, smooth colonies white, smooth colonies have a mix of fungi and bacteria the microscope, as. Trimethoprim and Vancomycin that it is a cocci-shaped bacterium measuring 0.5 to 0.7 μmin diameter Fig... Purified from the sputum of a species under the genus Planococcus have, I the Kraken app from... Extreme life styles that microbes could have, I that microbes could have, I test strip stain! State the genus Planococcus cells gram negative microbes these results are confirmation that the cells were prepped slides! Isolated from cyanobacterial mat samples collected from ponds in Antarctica showed partial acid-fast staining with the microscope such. Or from the slant agar susceptible to all antibiotics planococcus gram stain with the disk diffusion test was observed from growth the. A similar environment that confer certainty the microbe was susceptible to all antibiotics tested were Amikacin,,!
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